Hotspot

ndhE-ndhG

SSC · 118750-118960 · 211 bp

Back to Pterygiella

Pi 0.0142

MarkerSeek score 60.70

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 211
variable_sites: 3
pi: 0.0142
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	TCAAGACACATAAGCGCTCT	GGTGCAATTGCTATAGCTCG	45.0	50.0	57.0	57.0	339 bp	1.0	64.7
ndhE-ndhG_p2	AGTTCAAGACACATAAGCGCT	GGTGCAATTGCTATAGCTCG	42.9	50.0	58.0	57.0	342 bp	1.0	64.7
ndhE-ndhG_p3	ACACATAAGCGCTCTAACCA	GGTGCAATTGCTATAGCTCG	45.0	50.0	56.9	57.0	334 bp	1.0	64.7
ndhE-ndhG_p4	GACACATAAGCGCTCTAACCA	GGTGCAATTGCTATAGCTCG	47.6	50.0	58.1	57.0	335 bp	1.0	64.2
ndhE-ndhG_p5	AGACACATAAGCGCTCTAACC	GGTGCAATTGCTATAGCTCG	47.6	50.0	57.8	57.0	336 bp	1.0	63.7