Hotspot

rpl32-trnL-UAG

SSC · 114158-114959 · 802 bp

Back to Pterygiella

Pi 0.00623

MarkerSeek score 62.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 802
variable_sites: 5
pi: 0.00623
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGCAGCGTTAAAAGCGTTTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.5	57.9	926-927 bp	1.0	75.3
rpl32-trnL-UAG_p2	GGGTATTGGGCAGCGTTAAA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.5	57.9	934-935 bp	1.0	75.2
rpl32-trnL-UAG_p3	GGCAGCGTTAAAAGCGTTTT	AGCAGCGTGTCTACCAATTT	45.0	45.0	58.5	57.8	919-920 bp	1.0	74.8
rpl32-trnL-UAG_p4	GGCAGCGTTAAAAGCGTTTT	CCTAAGAGCAGCGTGTCTAC	45.0	55.0	58.5	57.8	925-926 bp	1.0	74.7
rpl32-trnL-UAG_p5	GGCAGCGTTAAAAGCGTTTT	CTAAGAGCAGCGTGTCTACC	45.0	55.0	58.5	57.8	924-925 bp	1.0	74.7