Hotspot

rps19-rpl2

IRb · 85331-85395 · 65 bp

Back to Pterygiella

Pi 0.0159

MarkerSeek score 64.50

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 65
variable_sites: 1
pi: 0.0159
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	GCGGGACCACGTTACTATTA	CATGGGGTTATCCTGCACTC	50.0	55.0	57.1	58.0	260-262 bp	1.0	73.7
rps19-rpl2_p2	GCGGGACCACGTTACTATTA	GGTTATCCTGCACTCGGAAG	50.0	55.0	57.1	58.1	255-257 bp	1.0	73.5
rps19-rpl2_p3	GCGGGACCACGTTACTATTA	GATTGTTCGTCGCCGTAGTA	50.0	50.0	57.1	57.8	199-201 bp	1.0	72.8
rps19-rpl2_p4	GCGGGACCACGTTACTATTA	TTTGATTGTTCGTCGCCGTA	50.0	45.0	57.1	58.2	202-204 bp	1.0	72.5
rps19-rpl2_p5	GCGGGACCACGTTACTATTA	AATTTGATTGTTCGTCGCCG	50.0	45.0	57.1	57.8	204-206 bp	1.0	72.3