Hotspot

psbT-psbN

LSC · 75584-75644 · 61 bp

Back to Pterygiella

Pi 0.0328

MarkerSeek score 71.40

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 61
variable_sites: 2
pi: 0.0328
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	CGAGAACCACCTAAAGTTCCA	GGGTACGCCTTATATACCGC	47.6	55.0	57.6	57.7	175 bp	1.0	67.5
psbT-psbN_p2	CGAGAACCACCTAAAGTTCCA	GTTTTACCGGGTACGCCTTA	47.6	50.0	57.6	57.6	183 bp	1.0	66.7
psbT-psbN_p3	CGAGAACCACCTAAAGTTCCA	TAAGTTTTACCGGGTACGCC	47.6	50.0	57.6	57.6	186 bp	1.0	66.7
psbT-psbN_p4	CGAGAACCACCTAAAGTTCCA	GATCCATTCGAGGAACACGG	47.6	55.0	57.6	58.4	127 bp	1.0	66.7
psbT-psbN_p5	CGAGAACCACCTAAAGTTCCA	CGGGTACGCCTTATATACCG	47.6	55.0	57.6	57.5	176 bp	1.0	65.3