Hotspot

trnP-UGG-psaJ

LSC · 67955-68357 · 403 bp

Back to Pterygiella

Pi 0
MarkerSeek score 30.30
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
403
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
30.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 ACCCGTGACATTTTGTACCC TGGGGCTACAGAGAGGTATG 50.0 55.0 58.1 57.9 494 bp 1.0 78.5
trnP-UGG-psaJ_p2 CCCGTGACATTTTGTACCCA TGGGGCTACAGAGAGGTATG 50.0 55.0 58.1 57.9 493 bp 1.0 78.5
trnP-UGG-psaJ_p3 ACCCGTGACATTTTGTACCC GGGGCTACAGAGAGGTATGT 50.0 55.0 58.1 57.9 493 bp 1.0 78.5
trnP-UGG-psaJ_p4 CCCGTGACATTTTGTACCCA GGGGCTACAGAGAGGTATGT 50.0 55.0 58.1 57.9 492 bp 1.0 78.5
trnP-UGG-psaJ_p5 ACAGGATTTGAACCCGTGAC TGGGGCTACAGAGAGGTATG 50.0 55.0 58.1 57.9 505 bp 1.0 78.4