Hotspot

petA-psbJ

LSC · 64410-65484 · 1075 bp

Back to Pterygiella

Pi 0.0131

MarkerSeek score 66.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1075
variable_sites: 14
pi: 0.0131
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTCCAAGGTCTTTTGT	TCCGGATTAGGTTCATCCCT	45.0	50.0	57.6	57.5	1205-1211 bp	1.0	74.1
petA-psbJ_p2	TCCATTACGTGTCCAAGGTC	TCCGGATTAGGTTCATCCCT	50.0	50.0	57.2	57.5	1211-1217 bp	1.0	71.4
petA-psbJ_p3	CCATTACGTGTCCAAGGTCT	TCCGGATTAGGTTCATCCCT	50.0	50.0	57.2	57.5	1210-1216 bp	1.0	71.3
petA-psbJ_p4	TCGAGAAGGTTCAATTGGCT	TCCGGATTAGGTTCATCCCT	45.0	50.0	57.1	57.5	1128-1134 bp	1.0	70.6
petA-psbJ_p5	CGAGAAGGTTCAATTGGCTG	TCCGGATTAGGTTCATCCCT	50.0	50.0	57.1	57.5	1127-1133 bp	1.0	70.2