Hotspot

rps16-trnQ-UUG

LSC · 6178-7386 · 1209 bp

Back to Pterygiella

Pi 0.00167

MarkerSeek score 60.30

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1209
variable_sites: 2
pi: 0.00167
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAAATTCAAATGGGGCGTGG	45.0	50.0	58.1	58.0	1340-1345 bp	1.0	79.4
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1282-1287 bp	1.0	78.9
rps16-trnQ-UUG_p3	AGCACCCTCATTCCTAGACA	GAAATTCAAATGGGGCGTGG	50.0	50.0	57.7	58.0	1405-1410 bp	1.0	78.5
rps16-trnQ-UUG_p4	AGCACCCTCATTCCTAGACA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.7	58.1	1347-1352 bp	1.0	78.0
rps16-trnQ-UUG_p5	CGTTGCTTTCTACCACATCG	GAAATTCAAATGGGGCGTGG	50.0	50.0	57.2	58.0	1332-1337 bp	1.0	74.2