Hotspot

ndhA

SSC · 128340-130468 · 2129 bp

Back to Pteroxygonum

Pi 0.0299

MarkerSeek score 63.00

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2129
variable_sites: 62
pi: 0.0299
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	AACCCGGTTACCATAGGGAA	AAAGGGCGAGTTGGGAATTT	50.0	45.0	58.0	58.0	2391-2439 bp	1.0	84.0
ndhA_p2	AACCCGGTTACCATAGGGAA	GGCATTATTGGCGGAGAAGA	50.0	50.0	58.0	58.0	2771-2819 bp	1.0	83.6
ndhA_p3	AACCCGGTTACCATAGGGAA	GGCGGAGAAGAGGCAATAAA	50.0	50.0	58.0	58.0	2762-2810 bp	1.0	83.5
ndhA_p4	AACCCGGTTACCATAGGGAA	AAGGGCGAGTTGGGAATTTT	50.0	45.0	58.0	58.0	2438 bp	0.5	59.7
ndhA_p5	AACCCGGTTACCATAGGGAA	AGGGCGAGTTGGGAATTTTT	50.0	45.0	58.0	58.0	2437 bp	0.5	59.7