Hotspot

rpl32-trnL-UAG

SSC · 122225-122758 · 534 bp

Back to Pteroxygonum

Pi 0.0625

MarkerSeek score 77.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 534
variable_sites: 32
pi: 0.0625
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AAAAGGGTATTCGGCAGCAT	TGGGTTGCTTCATTTGAGCT	45.0	45.0	57.9	58.0	1241 bp	0.5	58.8
rpl32-trnL-UAG_p2	AAAGGGTATTCGGCAGCATT	TGGGTTGCTTCATTTGAGCT	45.0	45.0	57.9	58.0	1240 bp	0.5	58.8
rpl32-trnL-UAG_p3	AAAAGGGTATTCGGCAGCAT	GTGGGTTGCTTCATTTGAGC	45.0	50.0	57.9	57.9	1242 bp	0.5	58.3
rpl32-trnL-UAG_p4	AAAGGGTATTCGGCAGCATT	GTGGGTTGCTTCATTTGAGC	45.0	50.0	57.9	57.9	1241 bp	0.5	58.3
rpl32-trnL-UAG_p5	AGAAAAGGGTATTCGGCAGC	TGGGTTGCTTCATTTGAGCT	50.0	45.0	58.2	58.0	1243 bp	0.5	58.0