Hotspot

ndhF-rpl32

SSC · 121063-122050 · 988 bp

Back to Pteroxygonum

Pi 0.0558
MarkerSeek score 78.60
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
988
variable_sites
54
pi
0.0558
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AGGTATGATCCATGCATATTGGT GCTTTTTGGAAATTGAAGTACGC 39.1 39.1 57.5 57.7 1064-1137 bp 1.0 45.4
ndhF-rpl32_p2 AGGTATGATCCATGCATATTGGT CGCTTTTTGGAAATTGAAGTACG 39.1 39.1 57.5 57.5 1065-1138 bp 1.0 45.4
ndhF-rpl32_p3 GGTATGATCCATGCATATTGGT GCTTTTTGGAAATTGAAGTACGC 40.9 39.1 56.0 57.7 1063-1136 bp 1.0 45.4
ndhF-rpl32_p4 AAGGTATGATCCATGCATATTGGT GCTTTTTGGAAATTGAAGTACGC 37.5 39.1 58.1 57.7 1065-1138 bp 1.0 45.4
ndhF-rpl32_p5 AGGTATGATCCATGCATATTGGT ACGAATACGCTTTTTGGAAA 39.1 35.0 57.5 54.1 1072-1145 bp 1.0 45.3