Hotspot

rpl2-rpl23

IRb · 89679-89697 · 19 bp

Back to Pteroxygonum

Pi 0.0556

MarkerSeek score 74.30

Primer available yes

Label rpl2-rpl23

Feature Metadata

label_name: rpl2-rpl23
feature_type: igs
region: IRb
length_bp: 19
variable_sites: 1
pi: 0.0556
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-rpl23_p1	TGACTGTCTACGGCCCTATT	TCCACCTCTTAGAAAGAAAAGAACT	50.0	36.0	57.8	57.9	108-109 bp	1.0	41.5
rpl2-rpl23_p2	TTGACTGTCTACGGCCCTAT	TCCACCTCTTAGAAAGAAAAGAACT	50.0	36.0	57.8	57.9	109-110 bp	1.0	41.5
rpl2-rpl23_p3	CCACAATGACGCTGTCCATA	TCCACCTCTTAGAAAGAAAAGAACT	50.0	36.0	58.0	57.9	159-160 bp	1.0	41.0
rpl2-rpl23_p4	TTACCACAATGACGCTGTCC	TCCACCTCTTAGAAAGAAAAGAACT	50.0	36.0	58.2	57.9	162-163 bp	1.0	41.0
rpl2-rpl23_p5	CCACAATGACGCTGTCCATA	TCCACCTCTTAGAAAGAAAAGAAC	50.0	37.5	58.0	56.6	159-160 bp	1.0	41.0