Hotspot

rps4-trnT-UGU

LSC · 48738-49431 · 694 bp

Back to Pteroxygonum

Pi 0.0874

MarkerSeek score 80.90

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 694
variable_sites: 47
pi: 0.0874
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	CCCCCAGACGTCGTATTTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.9	57.9	663-806 bp	1.0	87.2
rps4-trnT-UGU_p2	CCCCCAGACGTCGTATTTTT	GATGGTCATCGGTTCGACTC	50.0	55.0	57.9	58.2	632-775 bp	1.0	87.2
rps4-trnT-UGU_p3	TAAAACCCCCAGACGTCGTA	GATGGTCATCGGTTCGACTC	50.0	55.0	58.4	58.2	637-780 bp	1.0	85.1
rps4-trnT-UGU_p4	TAAAACCCCCAGACGTCGTA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.4	57.9	668-811 bp	1.0	85.0
rps4-trnT-UGU_p5	TAGTCCTGGTAAAACCCCCA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.6	57.9	677-820 bp	1.0	84.5