Hotspot

ycf3-trnS-GGA

LSC · 46789-47728 · 940 bp

Back to Pteroxygonum

Pi 0.0475

MarkerSeek score 79.50

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 940
variable_sites: 42
pi: 0.0475
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	GCCACAACTGAAAAGGTCTT	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.5	58.2	1036-1084 bp	1.0	73.6
ycf3-trnS-GGA_p2	GCCACAACTGAAAAGGTCTT	TTCCAATGCTACGCCTTGAA	45.0	45.0	56.5	57.8	986-1034 bp	1.0	73.4
ycf3-trnS-GGA_p3	GCCACAACTGAAAAGGTCTT	AGTTCCAATGCTACGCCTTG	45.0	50.0	56.5	58.5	988-1036 bp	1.0	70.6
ycf3-trnS-GGA_p4	GCCACAACTGAAAAGGTCTT	CAGTTCCAATGCTACGCCTT	45.0	50.0	56.5	58.5	989-1037 bp	1.0	70.6
ycf3-trnS-GGA_p5	GCCACAACTGAAAAGGTCTT	TCCAATGCTACGCCTTGAAC	45.0	50.0	56.5	58.6	985-1033 bp	1.0	70.6