Hotspot

trnE-UUC-trnT-GGU

LSC · 32811-33710 · 900 bp

Back to Pteroxygonum

Pi 0.046
MarkerSeek score 75.60
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
900
variable_sites
39
pi
0.046
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 CCAGCGTAGACATATTGCCA GAACCGATGACTTACGCCTT 50.0 50.0 57.8 58.0 1167-1183 bp 1.0 84.9
trnE-UUC-trnT-GGU_p2 ACCAGCGTAGACATATTGCC GAACCGATGACTTACGCCTT 50.0 50.0 57.8 58.0 1168-1184 bp 1.0 84.9
trnE-UUC-trnT-GGU_p3 TAGAGCGAATTATTGGGCCG GAACCGATGACTTACGCCTT 50.0 50.0 57.8 58.0 1200 bp 0.5 58.6
trnE-UUC-trnT-GGU_p4 TAGAGCGAATTATTGGGCCG CCATGGCGTTACTCTACCAC 50.0 55.0 57.8 58.1 1179 bp 0.5 58.2
trnE-UUC-trnT-GGU_p5 TAGAGCGAATTATTGGGCCG TGGCGTTACTCTACCACTGA 50.0 50.0 57.8 58.1 1176 bp 0.5 58.0