Hotspot

psbM-trnD-GUC

LSC · 30966-32104 · 1139 bp

Back to Pteroxygonum

Pi 0.0419

MarkerSeek score 75.70

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1139
variable_sites: 47
pi: 0.0419
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAAGAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.2	58.5	1242-1251 bp	1.0	57.7
psbM-trnD-GUC_p2	ACTAGAATGAAGAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	43.5	50.0	58.0	58.5	1245-1254 bp	1.0	56.2
psbM-trnD-GUC_p3	AGGAACTAGAATGAAGAGTGCAG	TTCAATTGGTCAGAGCACCG	43.5	50.0	57.9	58.5	1249-1258 bp	1.0	55.5
psbM-trnD-GUC_p4	AGGAACTAGAATGAAGAGTGCA	TTCAATTGGTCAGAGCACCG	40.9	50.0	56.8	58.5	1249-1258 bp	1.0	54.9
psbM-trnD-GUC_p5	GGAACTAGAATGAAGAGTGCAGT	TTCAATTGGTCAGAGCACCG	43.5	50.0	58.2	58.5	1248-1257 bp	1.0	54.8