Hotspot

trnR-UCU-atpA

LSC · 10788-10943 · 156 bp

Back to Pteroxygonum

Pi 0.0897

MarkerSeek score 76.60

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 156
variable_sites: 13
pi: 0.0897
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	ATGAGAAGCGTCCATTGTCT	GGAACGTTTTCTACTTCAGGA	45.0	42.9	56.9	55.6	250-260 bp	1.0	51.5
trnR-UCU-atpA_p2	ATCGTAATGAGAAGCGTCCA	GGAACGTTTTCTACTTCAGGA	45.0	42.9	56.8	55.6	256-266 bp	1.0	49.9
trnR-UCU-atpA_p3	TCGTAATGAGAAGCGTCCAT	GGAACGTTTTCTACTTCAGGA	45.0	42.9	56.8	55.6	255-265 bp	1.0	49.9
trnR-UCU-atpA_p4	ATGAGAAGCGTCCATTGTCT	GGAACGTTTTCTACTTCAGGAA	45.0	40.9	56.9	56.3	250-260 bp	1.0	49.5
trnR-UCU-atpA_p5	AATGAGAAGCGTCCATTGTCT	GGAACGTTTTCTACTTCAGGA	42.9	42.9	57.7	55.6	251-261 bp	1.0	49.3