Hotspot

matK

LSC · 2066-3589 · 1524 bp

Back to Pterostyrax

Pi 0
MarkerSeek score 40.00
Primer available yes
Label matK

Feature Metadata

label_name
matK
feature_type
gene
region
LSC
length_bp
1524
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK_p1 ACGCGGTCTCATAACAAATCA TGTTTTGACCGTATCGCACT 42.9 45.0 58.0 57.8 1621 bp 1.0 70.5
matK_p2 ACGCGGTCTCATAACAAATCA CCTTGTTTTGACCGTATCGC 42.9 50.0 58.0 57.5 1624 bp 1.0 67.6
matK_p3 CGCGGTCTCATAACAAATCA TGTTTTGACCGTATCGCACT 45.0 45.0 56.3 57.8 1620 bp 1.0 64.8
matK_p4 ACGCGGTCTCATAACAAATC TGTTTTGACCGTATCGCACT 45.0 45.0 56.3 57.8 1621 bp 1.0 64.7
matK_p5 ACGCGGTCTCATAACAAATCA TTGTTTTGACCGTATCGCAC 42.9 45.0 58.0 57.0 1622 bp 1.0 64.1