Hotspot

atpA

LSC · 11293-12816 · 1524 bp

Back to Pterostyrax

Pi 0
MarkerSeek score 40.00
Primer available yes
Label atpA

Feature Metadata

label_name
atpA
feature_type
gene
region
LSC
length_bp
1524
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpA_p1 AGAGAGGCTTCGTTACTCCT TGGTATGTTTGGAGCGATGA 50.0 45.0 57.5 56.9 1677-1678 bp 1.0 67.3
atpA_p2 CAGAGAGGCTTCGTTACTCC TGGTATGTTTGGAGCGATGA 55.0 45.0 57.4 56.9 1678-1679 bp 1.0 66.9
atpA_p3 AGAGAGGCTTCGTTACTCCT TGGTATGTTTGGAGCGATGAA 50.0 42.9 57.5 57.7 1677-1678 bp 1.0 65.2
atpA_p4 AGAGAGGCTTCGTTACTCCT TTGGTATGTTTGGAGCGATGA 50.0 42.9 57.5 57.7 1678-1679 bp 1.0 65.2
atpA_p5 CAGAGAGGCTTCGTTACTCC TGGTATGTTTGGAGCGATGAA 55.0 42.9 57.4 57.7 1678-1679 bp 1.0 64.7