Hotspot

atpA-atpF

LSC · 12817-12880 · 64 bp

Back to Pterostyrax

Pi 0

MarkerSeek score 54.00

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 64
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	GAATTTCGTCGGCTCGAATG	TGGTATGTTTGGAGCGATGA	50.0	45.0	57.6	56.9	128-129 bp	1.0	69.2
atpA-atpF_p2	ACTTGAAGTACGGTACCGGT	TGGTATGTTTGGAGCGATGA	50.0	45.0	58.4	56.9	204-205 bp	1.0	68.8
atpA-atpF_p3	TTGCTCAATACGTTCACGGA	TGGTATGTTTGGAGCGATGA	45.0	45.0	57.6	56.9	157-158 bp	1.0	68.4
atpA-atpF_p4	TCGCCTACTTGAAGTACGGT	TGGTATGTTTGGAGCGATGA	50.0	45.0	58.5	56.9	210-211 bp	1.0	68.1
atpA-atpF_p5	GAATTTCGTCGGCTCGAATG	TGGTATGTTTGGAGCGATGAA	50.0	42.9	57.6	57.7	128-129 bp	1.0	67.1