Hotspot

trnK-UUU-rps16

LSC · 4340-5202 · 863 bp

Back to Pterostyrax

Pi 0
MarkerSeek score 40.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
863
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CGAATCCCTTGCTTCATCCA AAGGGCGGAGGTTTTTAAGG 50.0 50.0 58.0 58.1 997 bp 1.0 79.0
trnK-UUU-rps16_p2 ACGAATCCCTTGCTTCATCC AAGGGCGGAGGTTTTTAAGG 50.0 50.0 58.0 58.1 998 bp 1.0 79.0
trnK-UUU-rps16_p3 ATGGACGAATCCCTTGCTTC AAGGGCGGAGGTTTTTAAGG 50.0 50.0 58.0 58.1 1002 bp 1.0 79.0
trnK-UUU-rps16_p4 GGACGAATCCCTTGCTTCAT AAGGGCGGAGGTTTTTAAGG 50.0 50.0 58.0 58.1 1000 bp 1.0 79.0
trnK-UUU-rps16_p5 CGAATCCCTTGCTTCATCCA GGGCGGAGGTTTTTAAGGAA 50.0 50.0 58.0 58.1 995 bp 1.0 79.0