Hotspot

matK-trnK-UUU

LSC · 3590-4302 · 713 bp

Back to Pterostyrax

Pi 0

MarkerSeek score 40.00

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 713
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	CGAAGCCGTATTGCTGAGAT	CGATTCGGGTTGCTAACTCA	50.0	50.0	58.1	58.0	811 bp	1.0	78.8
matK-trnK-UUU_p2	CGAAGCCGTATTGCTGAGAT	AACGGTAGAGTACTCGGCTT	50.0	50.0	58.1	58.2	792 bp	1.0	77.5
matK-trnK-UUU_p3	CGAAGCCGTATTGCTGAGAT	ACGGTAGAGTACTCGGCTTT	50.0	50.0	58.1	58.2	791 bp	1.0	77.5
matK-trnK-UUU_p4	CGAAGCCGTATTGCTGAGAT	TCGATTCGGGTTGCTAACTC	50.0	50.0	58.1	57.7	812 bp	1.0	76.6
matK-trnK-UUU_p5	CGAAGCCGTATTGCTGAGAT	GGGTTGCTAACTCAACGGTA	50.0	50.0	58.1	57.5	805 bp	1.0	75.2