Hotspot

rpl2

IRa · 161281-162797 · 1517 bp

Back to Pterospermum

Pi 0.00221

MarkerSeek score 51.30

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: IRa
length_bp: 1517
variable_sites: 5
pi: 0.00221
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	CGCTTCAACCGGGTTATTCT	TGTTTTTGTAAAGACGAAGAAAGA	50.0	29.2	58.3	55.5	1620-1633 bp	1.0	40.5
rpl2_p2	CGCTTCAACCGGGTTATTCT	AGACGAAGAAAGAAATTTTCTCTC	50.0	33.3	58.3	55.4	1609-1622 bp	1.0	40.5
rpl2_p3	ATCATTACGCTTCAACCGGG	TGTTTTTGTAAAGACGAAGAAAGA	50.0	29.2	58.3	55.5	1627-1640 bp	1.0	40.5
rpl2_p4	ATCATTACGCTTCAACCGGG	AGACGAAGAAAGAAATTTTCTCTC	50.0	33.3	58.3	55.4	1616-1629 bp	1.0	40.5
rpl2_p5	CGCTTCAACCGGGTTATTCT	AGACGAAGAAAGAAATTTTCTCT	50.0	30.4	58.3	54.3	1609-1622 bp	1.0	40.5