Hotspot

trnN-GUU-ndhF

IRb · 116433-116979 · 547 bp

Back to Pterospermum

Pi 0.0243

MarkerSeek score 50.00

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 547
variable_sites: 19
pi: 0.0243
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 50.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	GGGTTACCTGCTCTTAACGG	ATATGTAGGGGGAAGTCGCA	55.0	50.0	58.0	57.9	784-794 bp	1.0	83.7
trnN-GUU-ndhF_p2	GGGTTACCTGCTCTTAACGG	TATGTAGGGGGAAGTCGCAT	55.0	50.0	58.0	57.9	783-793 bp	1.0	83.7
trnN-GUU-ndhF_p3	GGGTTACCTGCTCTTAACGG	GGGGAAGTCGCATCTCTTTT	55.0	50.0	58.0	57.9	776-786 bp	1.0	83.6
trnN-GUU-ndhF_p4	GGCAGAATAGCAGAGCAAGT	ATATGTAGGGGGAAGTCGCA	50.0	50.0	58.0	57.9	1090-1100 bp	1.0	82.3
trnN-GUU-ndhF_p5	GGCAGAATAGCAGAGCAAGT	TATGTAGGGGGAAGTCGCAT	50.0	50.0	58.0	57.9	1089-1099 bp	1.0	82.3