Hotspot

rpl33-rps18

LSC · 73839-74421 · 583 bp

Back to Pterospermum

Pi 0.028

MarkerSeek score 66.10

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 583
variable_sites: 22
pi: 0.028
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CGACACAATACGCCTAGTCG	TCAATTCGATCCCCCGATTG	55.0	50.0	58.5	57.7	756-774 bp	1.0	80.0
rpl33-rps18_p2	GACACAATACGCCTAGTCGA	TCAATTCGATCCCCCGATTG	50.0	50.0	57.2	57.7	755-773 bp	1.0	77.6
rpl33-rps18_p3	TCGACACAATACGCCTAGTC	TCAATTCGATCCCCCGATTG	50.0	50.0	57.2	57.7	757-775 bp	1.0	77.5
rpl33-rps18_p4	CGACACAATACGCCTAGTCG	ATTCGATCCCCCGATTGGAT	55.0	50.0	58.5	58.6	753-771 bp	1.0	76.9
rpl33-rps18_p5	ACGCCTAGTCGATTGGAATT	TCAATTCGATCCCCCGATTG	45.0	50.0	57.0	57.7	747-765 bp	1.0	76.4