Hotspot

psaJ-rpl33

LSC · 73116-73637 · 522 bp

Back to Pterospermum

Pi 0.00534

MarkerSeek score 52.00

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 522
variable_sites: 4
pi: 0.00534
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTCCCGGATGCGTTAACATT	ATACCTGGAAATGCCCGTTG	45.0	50.0	57.9	58.2	631-654 bp	1.0	80.5
psaJ-rpl33_p2	GTTTCTTCCCGGATGCGTTA	ATACCTGGAAATGCCCGTTG	50.0	50.0	58.3	58.2	636-659 bp	1.0	79.3
psaJ-rpl33_p3	TTCTTCCCGGATGCGTTAAC	ATACCTGGAAATGCCCGTTG	50.0	50.0	58.3	58.2	634-657 bp	1.0	79.3
psaJ-rpl33_p4	CGGATGCGTTAACATTCCCT	ATACCTGGAAATGCCCGTTG	50.0	50.0	58.3	58.2	627-650 bp	1.0	78.8
psaJ-rpl33_p5	CTTCCCGGATGCGTTAACAT	ATACCTGGAAATGCCCGTTG	50.0	50.0	58.3	58.2	632-655 bp	1.0	78.8