Hotspot

ndhC-trnM-CAU

LSC · 55489-57466 · 1978 bp

Back to Pterospermum

Pi 0.00698
MarkerSeek score 57.60
Primer available yes
Label ndhC-trnM-CAU

Feature Metadata

label_name
ndhC-trnM-CAU
feature_type
igs
region
LSC
length_bp
1978
variable_sites
17
pi
0.00698
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnM-CAU_p1 ACCCTTTCTAATCGGGGCTA AGACACCGGGATCAATGGTA 50.0 50.0 57.8 58.1 2194-2225 bp 1.0 79.2
ndhC-trnM-CAU_p2 ACCCTTTCTAATCGGGGCTA TAGACACCGGGATCAATGGT 50.0 50.0 57.8 58.1 2195-2226 bp 1.0 79.2
ndhC-trnM-CAU_p3 CGGGGCTAAAACTCCAGAAA AGACACCGGGATCAATGGTA 50.0 50.0 57.8 58.1 2182-2213 bp 1.0 79.2
ndhC-trnM-CAU_p4 CGGGGCTAAAACTCCAGAAA TAGACACCGGGATCAATGGT 50.0 50.0 57.8 58.1 2183-2214 bp 1.0 79.2
ndhC-trnM-CAU_p5 ATCGGGGCTAAAACTCCAGA AGACACCGGGATCAATGGTA 50.0 50.0 58.4 58.1 2184-2215 bp 1.0 77.4