Hotspot

rps4-trnT-UGU

LSC · 49991-50735 · 745 bp

Back to Pterospermum

Pi 0.0448
MarkerSeek score 69.20
Primer available yes
Label rps4-trnT-UGU

Feature Metadata

label_name
rps4-trnT-UGU
feature_type
igs
region
LSC
length_bp
745
variable_sites
49
pi
0.0448
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnT-UGU_p1 TAAGATCGCTTCCGGCTCTA CTCAGAGGTTAGAGCATCGC 50.0 55.0 58.0 57.9 905-971 bp 1.0 84.2
rps4-trnT-UGU_p2 TTTCTAAGATCGCTTCCGGC CTCAGAGGTTAGAGCATCGC 50.0 55.0 58.1 57.9 909-975 bp 1.0 83.9
rps4-trnT-UGU_p3 TAAGATCGCTTCCGGCTCTA TTGAGTTTGAGCCCGCTTAG 50.0 50.0 58.0 58.2 925-991 bp 1.0 83.7
rps4-trnT-UGU_p4 TAAGATCGCTTCCGGCTCTA ATGGTCATCGGTTCGATTCC 50.0 50.0 58.0 57.8 873-939 bp 1.0 83.6
rps4-trnT-UGU_p5 TTTCTAAGATCGCTTCCGGC TTGAGTTTGAGCCCGCTTAG 50.0 50.0 58.1 58.2 929-995 bp 1.0 83.3