Hotspot

rps16-trnQ-UUG

LSC · 7065-7634 · 570 bp

Back to Pterospermum

Pi 0.0183

MarkerSeek score 52.40

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 570
variable_sites: 15
pi: 0.0183
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AGTGGTGGGTTTTGACATCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	686-780 bp	1.0	83.5
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	643-737 bp	1.0	82.7
rps16-trnQ-UUG_p3	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	654-748 bp	1.0	82.1
rps16-trnQ-UUG_p4	TTTTGACATCCACAGCCGAT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.8	58.1	677-771 bp	1.0	82.0
rps16-trnQ-UUG_p5	TTTGACATCCACAGCCGATC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.3	58.1	676-770 bp	1.0	81.5