Hotspot

psbA-trnK-UUU

LSC · 1833-2389 · 557 bp

Back to Pterospermum

Pi 0.0545

MarkerSeek score 77.10

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 557
variable_sites: 42
pi: 0.0545
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	AACACCAAACCATCCGATGT	CGGTTTGGGGAGGGATTTTT	45.0	50.0	57.7	58.4	768-769 bp	1.0	83.2
psbA-trnK-UUU_p2	ACGGTTTTCAGTGCTGGTTA	CGGTTTGGGGAGGGATTTTT	45.0	50.0	57.7	58.4	744-745 bp	1.0	82.9
psbA-trnK-UUU_p3	AACACCAAACCATCCGATGT	AAAATGCAAGCACGGTTTGG	45.0	45.0	57.7	58.4	780-781 bp	1.0	82.7
psbA-trnK-UUU_p4	ACGGTTTTCAGTGCTGGTTA	AAAATGCAAGCACGGTTTGG	45.0	45.0	57.7	58.4	756-757 bp	1.0	82.4
psbA-trnK-UUU_p5	AACACCAAACCATCCGATGT	ACTCCATCCGACTAGTTCCG	45.0	55.0	57.7	58.6	704-705 bp	1.0	81.6