Hotspot

rps8

LSC · 84857-85267 · 411 bp

Back to Pterodon

Pi 0

MarkerSeek score 34.40

Primer available yes

Label rps8

Feature Metadata

label_name: rps8
feature_type: gene
region: LSC
length_bp: 411
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8_p1	ACTTCCTTCACGTATTCACT	TCGAAAGTCAGAACCAAGAA	40.0	40.0	54.0	54.6	564 bp	1.0	40.0
rps8_p2	AACTTCCTTCACGTATTCACT	TCGAAAGTCAGAACCAAGAA	38.1	40.0	54.9	54.6	565 bp	1.0	40.0
rps8_p3	ACTTCCTTCACGTATTCACT	TCGAAAGTCAGAACCAAGAAA	40.0	38.1	54.0	55.4	564 bp	1.0	40.0
rps8_p4	ACTTCCTTCACGTATTCACT	ATCGAAAGTCAGAACCAAGA	40.0	40.0	54.0	54.3	565 bp	1.0	40.0
rps8_p5	AACTTCCTTCACGTATTCACT	TCGAAAGTCAGAACCAAGAAA	38.1	38.1	54.9	55.4	565 bp	1.0	40.0