Hotspot

rps8-rpl14

LSC · 85268-85526 · 259 bp

Back to Pterodon

Pi 0.0463

MarkerSeek score 79.10

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 259
variable_sites: 12
pi: 0.0463
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	ATTACGTCAGCGATGGTGTC	AATTTTTGGTGCAATCGCCC	50.0	45.0	58.1	58.2	367 bp	1.0	80.5
rps8-rpl14_p2	GTCAGCGATGGTGTCCTTAC	AATTTTTGGTGCAATCGCCC	55.0	45.0	58.4	58.2	362 bp	1.0	78.2
rps8-rpl14_p3	ATTACGTCAGCGATGGTGTC	CGAATTTTTGGTGCAATCGC	50.0	45.0	58.1	57.2	369 bp	1.0	75.8
rps8-rpl14_p4	ATTACGTCAGCGATGGTGTC	GCCCGGGAATTGAAACAGTT	50.0	50.0	58.1	59.0	351 bp	1.0	73.8
rps8-rpl14_p5	GTCAGCGATGGTGTCCTTAC	CGAATTTTTGGTGCAATCGC	55.0	45.0	58.4	57.2	364 bp	1.0	73.6