Hotspot

accD-psaI

LSC · 62115-62679 · 565 bp

Back to Pterodon

Pi 0.00885

MarkerSeek score 63.10

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 565
variable_sites: 5
pi: 0.00885
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TTGATTCAATCGTACCGCGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	727-730 bp	1.0	80.3
accD-psaI_p2	GCGTAGTCCTTTAAAGGGGG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	711-714 bp	1.0	80.1
accD-psaI_p3	TTGATTCAATCGTACCGCGT	AAGAAGCCATTGCAATTGCC	45.0	45.0	58.0	57.9	733-736 bp	1.0	79.7
accD-psaI_p4	GCGTAGTCCTTTAAAGGGGG	AAGAAGCCATTGCAATTGCC	55.0	45.0	58.0	57.9	717-720 bp	1.0	79.5
accD-psaI_p5	TTCAATCGTACCGCGTAGTC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	723-726 bp	1.0	78.9