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atpI-atpH

LSC · 49226-50592 · 1367 bp

Back to Pterodon

Pi 0.00293

MarkerSeek score 60.40

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 1367
variable_sites: 4
pi: 0.00293
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	GCTGCGGCTTATATAGGTGA	GATCCCTTCTACAGCTTGCC	50.0	55.0	57.3	58.0	1526 bp	1.0	73.9
atpI-atpH_p2	ACAAGCGGTATTCAAGCTCT	GATCCCTTCTACAGCTTGCC	45.0	55.0	57.2	58.0	1562 bp	1.0	73.7
atpI-atpH_p3	TAGGTGAGTCCATGGAAGGC	GATCCCTTCTACAGCTTGCC	55.0	55.0	58.8	58.0	1513 bp	1.0	73.5
atpI-atpH_p4	GCTGCGGCTTATATAGGTGA	AACGGAAGCAGCAGAAATCA	50.0	45.0	57.3	58.1	1439 bp	1.0	73.4
atpI-atpH_p5	GCTGCGGCTTATATAGGTGA	GTCCAATAGAAGCAAGCCCA	50.0	50.0	57.3	57.9	1479 bp	1.0	73.2