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trnK-UUU-rbcL

LSC · 4301-5550 · 1250 bp

Back to Pterodon

Pi 0.00881

MarkerSeek score 64.00

Primer available yes

Label trnK-UUU-rbcL

Feature Metadata

label_name: trnK-UUU-rbcL
feature_type: igs
region: LSC
length_bp: 1250
variable_sites: 11
pi: 0.00881
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rbcL_p1	AAAGCCGAGTACTCTACCGT	AGTCCTGAATTAGCTGCTGC	50.0	50.0	58.2	58.0	1359 bp	1.0	79.1
trnK-UUU-rbcL_p2	AAGCCGAGTACTCTACCGTT	AGTCCTGAATTAGCTGCTGC	50.0	50.0	58.2	58.0	1358 bp	1.0	79.1
trnK-UUU-rbcL_p3	AAAGCCGAGTACTCTACCGT	GAGGCTAGCAAATGGAGTCC	50.0	55.0	58.2	58.0	1374 bp	1.0	79.1
trnK-UUU-rbcL_p4	AAGCCGAGTACTCTACCGTT	GAGGCTAGCAAATGGAGTCC	50.0	55.0	58.2	58.0	1373 bp	1.0	79.1
trnK-UUU-rbcL_p5	AAGCCGAGTACTCTACCGTT	GGCTAGCAAATGGAGTCCTG	50.0	55.0	58.2	58.3	1371 bp	1.0	76.8