Hotspot

petB

LSC · 77564-78961 · 1398 bp

Back to Pterocyclus

Pi 0.00787

MarkerSeek score 51.00

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1398
variable_sites: 22
pi: 0.00787
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	TGTTGAGAAGGTTGCCCAAA	GTGCTTTCTGGGTTGTCTCA	45.0	50.0	57.8	58.0	2600-2602 bp	1.0	79.6
petB_p2	TTGTTGAGAAGGTTGCCCAA	GTGCTTTCTGGGTTGTCTCA	45.0	50.0	57.8	58.0	2601-2603 bp	1.0	79.6
petB_p3	TGTTCCTCGAACGGATCTCT	GTGCTTTCTGGGTTGTCTCA	50.0	50.0	58.2	58.0	2624-2626 bp	1.0	79.5
petB_p4	TGTTGAGAAGGTTGCCCAAA	TCTGGGTTGTCTCAGACCTT	45.0	50.0	57.8	57.9	2594-2596 bp	1.0	79.0
petB_p5	TGTTGAGAAGGTTGCCCAAA	TTCTGGGTTGTCTCAGACCT	45.0	50.0	57.8	57.9	2595-2597 bp	1.0	79.0