Hotspot

ndhH

SSC · 125452-126633 · 1182 bp

Back to Pterocyclus

Pi 0.00212

MarkerSeek score 60.20

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 5
pi: 0.00212
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	AGGGACAAGCATCCATATGA	CGCTTTCAGCAATTCATGGA	45.0	45.0	56.0	57.1	1321 bp	1.0	56.6
ndhH_p2	AGGGACAAGCATCCATATGA	TCGCTTTCAGCAATTCATGG	45.0	45.0	56.0	57.1	1322 bp	1.0	56.6
ndhH_p3	AGGGACAAGCATCCATATGATC	CGCTTTCAGCAATTCATGGA	45.5	45.0	57.7	57.1	1321 bp	1.0	54.5
ndhH_p4	GGGACAAGCATCCATATGATCT	CGCTTTCAGCAATTCATGGA	45.5	45.0	57.7	57.1	1320 bp	1.0	54.5
ndhH_p5	GGGACAAGCATCCATATGATCT	TCGCTTTCAGCAATTCATGG	45.5	45.0	57.7	57.1	1321 bp	1.0	54.5