Hotspot

ndhG-ndhI

SSC · 122218-122669 · 452 bp

Back to Pterocyclus

Pi 0.0281

MarkerSeek score 63.70

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 452
variable_sites: 20
pi: 0.0281
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	GCAATCGCACCTATTAACGC	TTCTTTGGGGCGTTTACCAA	50.0	45.0	58.0	57.9	998-1059 bp	1.0	85.5
ndhG-ndhI_p2	GCAATCGCACCTATTAACGC	TTTCTTTGGGGCGTTTACCA	50.0	45.0	58.0	57.9	999-1060 bp	1.0	85.5
ndhG-ndhI_p3	GCAATCGCACCTATTAACGC	AACAAACAGTACGAGCTGCA	50.0	45.0	58.0	58.1	1380-1441 bp	1.0	85.1
ndhG-ndhI_p4	GCAATCGCACCTATTAACGC	ACAAACAGTACGAGCTGCAA	50.0	45.0	58.0	58.1	1379-1440 bp	1.0	85.1
ndhG-ndhI_p5	CATTTATGGCCCCCACGTAA	ACAAACAGTACGAGCTGCAA	50.0	45.0	57.9	58.1	1078-1139 bp	1.0	85.0