Hotspot

rpl32-trnL-UAG

SSC · 116683-117668 · 986 bp

Back to Pterocyclus

Pi 0.0137

MarkerSeek score 67.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 986
variable_sites: 23
pi: 0.0137
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GCAGCCTTAAAAGCGTTGTC	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.3	58.0	1068-1102 bp	1.0	81.7
rpl32-trnL-UAG_p2	GCAGCCTTAAAAGCGTTGTC	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.3	58.0	1069-1103 bp	1.0	81.7
rpl32-trnL-UAG_p3	GAAAGGATATTGGGCAGCCT	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.6	58.0	1081-1115 bp	1.0	81.2
rpl32-trnL-UAG_p4	GAAAGGATATTGGGCAGCCT	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.6	58.0	1082-1116 bp	1.0	81.2
rpl32-trnL-UAG_p5	GCAGCCTTAAAAGCGTTGTC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.3	57.9	1078-1112 bp	1.0	80.7