Hotspot

trnK-UUU-rps16

LSC · 4480-5362 · 883 bp

Back to Pterocyclus

Pi 0.0408
MarkerSeek score 78.70
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
883
variable_sites
70
pi
0.0408
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCTTTCAGGATCAGTCGTGG GCCGTACGAGGAGAAAACTT 55.0 50.0 58.0 57.9 859-1280 bp 1.0 88.2
trnK-UUU-rps16_p2 CCTTTCAGGATCAGTCGTGG GGGGAATCGAGCGTTTGTAT 55.0 50.0 58.0 58.1 859-1748 bp 1.0 87.1
trnK-UUU-rps16_p3 TCCCTCGCTTCATCCAAATG AAAGTGTATCGCGGGGAATC 50.0 50.0 58.0 58.1 809-1709 bp 1.0 87.1
trnK-UUU-rps16_p4 TCCCTCGCTTCATCCAAATG GGGGAATCGAGCGTTTGTAT 50.0 50.0 58.0 58.1 808-1697 bp 1.0 87.1
trnK-UUU-rps16_p5 CCTTTCAGGATCAGTCGTGG AAAGTGTATCGCGGGGAATC 55.0 50.0 58.0 58.1 860-1760 bp 1.0 87.0