Hotspot

trnH-GUG-psbA

LSC · 232-606 · 375 bp

Back to Pterocyclus

Pi 0.0124

MarkerSeek score 54.60

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 375
variable_sites: 8
pi: 0.0124
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	ACCGCGCTAATCTTGGTATG	55.0	50.0	58.1	58.1	496-503 bp	1.0	79.3
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	ACCGCGCTAATCTTGGTATG	50.0	50.0	57.9	58.1	510-517 bp	1.0	79.3
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	ACCGCGCTAATCTTGGTATG	50.0	50.0	57.9	58.1	509-516 bp	1.0	79.3
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	ACCGCGCTAATCTTGGTATG	50.0	50.0	57.9	58.1	511-518 bp	1.0	79.2
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	CGCGCTAATCTTGGTATGGA	55.0	50.0	58.1	57.9	494-501 bp	1.0	79.2