Hotspot

rps2

LSC · 17592-18302 · 711 bp

Back to Pterocarya

Pi 0.00159

MarkerSeek score 36.00

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 711
variable_sites: 4
pi: 0.00159
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	ACTTGTATCTCTTTTGCGCG	AAAGAGAGGAAGTGTGGGGA	45.0	50.0	56.8	57.9	839 bp	1.0	70.4
rps2_p2	ACTTGTATCTCTTTTGCGCGA	AAAGAGAGGAAGTGTGGGGA	42.9	50.0	58.3	57.9	839 bp	1.0	69.2
rps2_p3	ACTTGTATCTCTTTTGCGCG	AAGAGAGGAAGTGTGGGGAG	45.0	55.0	56.8	58.3	838 bp	1.0	68.6
rps2_p4	ACTTGTATCTCTTTTGCGCG	GAGGAAGTGTGGGGAGAGAA	45.0	55.0	56.8	58.4	834 bp	1.0	68.5
rps2_p5	CTTGTATCTCTTTTGCGCGA	AAAGAGAGGAAGTGTGGGGA	45.0	50.0	56.6	57.9	838 bp	1.0	68.2