Hotspot

psbA

LSC · 303-1364 · 1062 bp

Back to Pterocarya

Pi 0.000565

MarkerSeek score 46.20

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 3
pi: 0.000565
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	GGAGCAATACCAATCCTCTTGA	TGCTTGGGAGTCCCTAATGA	45.5	50.0	57.8	58.0	1190-1197 bp	1.0	63.1
psbA_p2	AGGAGCAATACCAATCCTCTTG	TGCTTGGGAGTCCCTAATGA	45.5	50.0	57.8	58.0	1191-1198 bp	1.0	63.1
psbA_p3	GGAGCAATACCAATCCTCTTGA	GTGCTTGGGAGTCCCTAATG	45.5	55.0	57.8	58.0	1191-1198 bp	1.0	63.1
psbA_p4	AGGAGCAATACCAATCCTCTTG	GTGCTTGGGAGTCCCTAATG	45.5	55.0	57.8	58.0	1192-1199 bp	1.0	63.0
psbA_p5	GGAGCAATACCAATCCTCTTGA	AGAATTCGTGTGCTTGGGAG	45.5	50.0	57.8	58.2	1200-1207 bp	1.0	61.9