Hotspot

ndhF

SSC · 115897-118122 · 2226 bp

Back to Pterocarya

Pi 0.00866

MarkerSeek score 49.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2226
variable_sites: 70
pi: 0.00866
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GAGGATCCGGACAAAATGGA	TGGGGTTAAGTTATGGACTGTG	50.0	45.5	57.3	57.7	2902-2996 bp	1.0	61.6
ndhF_p2	GAGGATCCGGACAAAATGGA	GGGGTTAAGTTATGGACTGTGT	50.0	45.5	57.3	57.7	2901-2995 bp	1.0	61.6
ndhF_p3	GAGGATCCGGACAAAATGGA	CTGGGGTTAAGTTATGGACTGT	50.0	45.5	57.3	57.4	2903-2997 bp	1.0	59.4
ndhF_p4	AGGATCCGGACAAAATGGATG	TGGGGTTAAGTTATGGACTGTG	47.6	45.5	58.1	57.7	2901-2995 bp	1.0	58.8
ndhF_p5	AGGATCCGGACAAAATGGATG	GGGGTTAAGTTATGGACTGTGT	47.6	45.5	58.1	57.7	2900-2994 bp	1.0	58.8