Hotspot

ycf1-ndhF

SSC · 115842-115896 · 55 bp

Back to Pterocarya

Pi 0.163

MarkerSeek score 67.60

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 55
variable_sites: 26
pi: 0.163
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	ACTTCCGAAACAAAGGGGAC	ACTTTGTTCGTTGGAGCCAT	50.0	45.0	58.0	58.0	982-1075 bp	1.0	91.7
ycf1-ndhF_p2	ACTTCCGAAACAAAGGGGAC	TTGTTCGTTGGAGCCATAGG	50.0	50.0	58.0	57.9	979-1072 bp	1.0	91.0
ycf1-ndhF_p3	CGGAAGAAAGCGAGGAAGAA	ACTTTGTTCGTTGGAGCCAT	50.0	45.0	57.9	58.0	1023-1116 bp	0.3	59.7
ycf1-ndhF_p4	CGGAAGAAAGCGAGGAAGAA	TTGTTCGTTGGAGCCATAGG	50.0	50.0	57.9	57.9	1020-1113 bp	0.3	59.0
ycf1-ndhF_p5	AACGGAAGAAAGCGAGGAAG	ACTTTGTTCGTTGGAGCCAT	50.0	45.0	58.2	58.0	1025-1118 bp	0.3	58.7