Hotspot

accD-psaI

LSC · 63912-64694 · 783 bp

Back to Pterocarya

Pi 0.004

MarkerSeek score 64.20

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 783
variable_sites: 10
pi: 0.004
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GATCCAATCGTACCGCGTAA	AAAGAAGCCATCGCAATTGC	50.0	45.0	57.9	58.0	951-985 bp	1.0	81.0
accD-psaI_p2	TCCAATCGTACCGCGTAATC	AAAGAAGCCATCGCAATTGC	50.0	45.0	57.9	58.0	949-983 bp	1.0	81.0
accD-psaI_p3	CCAATCGTACCGCGTAATCT	AAAGAAGCCATCGCAATTGC	50.0	45.0	57.9	58.0	948-982 bp	1.0	81.0
accD-psaI_p4	GATCCAATCGTACCGCGTAA	TCGCAATTGCCGGAAATACT	50.0	45.0	57.9	58.3	941-975 bp	1.0	79.0
accD-psaI_p5	TCCAATCGTACCGCGTAATC	TCGCAATTGCCGGAAATACT	50.0	45.0	57.9	58.3	939-973 bp	1.0	79.0