Hotspot

trnF-GAA-ndhJ

LSC · 53266-54096 · 831 bp

Back to Pterocarya

Pi 0.00302

MarkerSeek score 58.60

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 831
variable_sites: 9
pi: 0.00302
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AATGCCGGAAAGCTGGATAG	55.0	50.0	58.0	58.0	935-1012 bp	1.0	84.5
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AATGCCGGAAAGCTGGATAG	50.0	50.0	58.1	58.0	899-976 bp	1.0	84.1
trnF-GAA-ndhJ_p3	TGATACGCCAGAAATGGTCG	AATGCCGGAAAGCTGGATAG	50.0	50.0	58.1	58.0	954-1031 bp	1.0	84.0
trnF-GAA-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	GAAAGCTGGATAGGATGGCC	55.0	55.0	58.0	58.1	928-1005 bp	1.0	83.9
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	GGAAAGCTGGATAGGATGGC	55.0	55.0	58.0	58.1	929-1006 bp	1.0	83.9