Hotspot

lhbA-trnG-UCC

LSC · 40419-40854 · 436 bp

Back to Pterocarya

Pi 0.0068
MarkerSeek score 63.10
Primer available yes
Label lhbA-trnG-UCC

Feature Metadata

label_name
lhbA-trnG-UCC
feature_type
igs
region
LSC
length_bp
436
variable_sites
9
pi
0.0068
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.97
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
lhbA-trnG-UCC_p1 CTTCTCCTGATGGTTGGTCG ATTGAACCCGCATCTTCTCC 55.0 50.0 58.0 58.0 475-618 bp 1.0 84.8
lhbA-trnG-UCC_p2 TTGCTTCTCCTGATGGTTGG ATTGAACCCGCATCTTCTCC 50.0 50.0 57.8 58.0 478-621 bp 1.0 83.3
lhbA-trnG-UCC_p3 TCCTGATGGTTGGTCGAGTA ATTGAACCCGCATCTTCTCC 50.0 50.0 57.8 58.0 471-614 bp 1.0 83.2
lhbA-trnG-UCC_p4 GCTTCTCCTGATGGTTGGTC ATTGAACCCGCATCTTCTCC 55.0 50.0 58.3 58.0 476-619 bp 1.0 82.9
lhbA-trnG-UCC_p5 TTCTCCTGATGGTTGGTCGA ATTGAACCCGCATCTTCTCC 50.0 50.0 58.4 58.0 474-617 bp 1.0 82.1