Hotspot

trnS-UGA-lhbA

LSC · 39817-40229 · 413 bp

Back to Pterocarya

Pi 0.000861
MarkerSeek score 37.80
Primer available yes
Label trnS-UGA-lhbA

Feature Metadata

label_name
trnS-UGA-lhbA
feature_type
igs
region
LSC
length_bp
413
variable_sites
1
pi
0.000861
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
37.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-UGA-lhbA_p1 CAAGACCGGAGCTATCAACC CGACCAACCATCAGGAGAAG 55.0 55.0 58.1 58.0 539-565 bp 1.0 81.3
trnS-UGA-lhbA_p2 CAAGACCGGAGCTATCAACC CGCAAAAACAGCCAATTGGA 55.0 45.0 58.1 58.1 470-496 bp 1.0 80.6
trnS-UGA-lhbA_p3 CAAGACCGGAGCTATCAACC CCAACCATCAGGAGAAGCAA 55.0 50.0 58.1 57.8 536-562 bp 1.0 79.8
trnS-UGA-lhbA_p4 CAAGACCGGAGCTATCAACC TACTCGACCAACCATCAGGA 55.0 50.0 58.1 57.8 543-569 bp 1.0 79.6
trnS-UGA-lhbA_p5 CAAGACCGGAGCTATCAACC GACCAACCATCAGGAGAAGC 55.0 55.0 58.1 58.3 538-564 bp 1.0 79.4