Hotspot

trnM-CAU-psbD

LSC · 35516-37050 · 1535 bp

Back to Pterocarya

Pi 0.00259

MarkerSeek score 66.50

Primer available yes

Label trnM-CAU-psbD

Feature Metadata

label_name: trnM-CAU-psbD
feature_type: igs
region: LSC
length_bp: 1535
variable_sites: 14
pi: 0.00259
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-psbD_p1	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1665-1696 bp	1.0	81.7
trnM-CAU-psbD_p2	GTGGTAGAGTAACGCCATGG	GACCGGACCAACCTACAAAA	55.0	50.0	58.1	57.7	1679-1710 bp	1.0	81.5
trnM-CAU-psbD_p3	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1668-1699 bp	1.0	81.5
trnM-CAU-psbD_p4	TCAGTGGTAGAGTAACGCCA	GACCGGACCAACCTACAAAA	50.0	50.0	58.1	57.7	1682-1713 bp	1.0	81.3
trnM-CAU-psbD_p5	GTGGTAGAGTAACGCCATGG	TAGACCGGACCAACCTACAA	55.0	50.0	58.1	57.7	1681-1712 bp	1.0	81.1