Hotspot

trnD-GUC-trnY-GUA

LSC · 33668-34182 · 515 bp

Back to Pterocarya

Pi 0.00561
MarkerSeek score 53.10
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
515
variable_sites
9
pi
0.00561
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 CGGTGCTCTGACCAATTGAA TGGCAATATGTCTACGCTGG 50.0 50.0 58.5 57.8 574-599 bp 1.0 77.4
trnD-GUC-trnY-GUA_p2 CGGTGCTCTGACCAATTGAA GGCAATATGTCTACGCTGGT 50.0 50.0 58.5 57.8 573-598 bp 1.0 77.4
trnD-GUC-trnY-GUA_p3 CGGTGCTCTGACCAATTGAA CGGACTGTAAATTCGTTGGC 50.0 50.0 58.5 57.5 590-615 bp 1.0 75.1
trnD-GUC-trnY-GUA_p4 CGGTGCTCTGACCAATTGAA GCGGACTGTAAATTCGTTGG 50.0 50.0 58.5 57.5 591-616 bp 1.0 75.1
trnD-GUC-trnY-GUA_p5 CGGTGCTCTGACCAATTGAA GGCGGACTGTAAATTCGTTG 50.0 50.0 58.5 57.5 592-617 bp 1.0 75.1